circRNA basic information
circBase ID: hsa_circ_0014235
Name: hsa_circ_S100A2
Synonym: -
Host Gene: S100A2
Genomic location(hg19): chr1:153536209-153536363:-
Genomic location(hg38): chr1:153563733-153563887:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

serum-derived exosomes; tumor tissues; adjacent normal tissues

Cell lines:

A549; H1299; 16HBE; 293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: cyclin-dependent kinase 4 (CDK4)
Associated microRNA: miR-520a-5p
Biological function: Promotes cisplatin (DDP) chemoresistance; promotes proliferation, colony formation, cell cycle progression, migration and invasion; inhibits apoptosis; promotes tumor growth in vivo.
Molecular mechanism: Exosome-transferred hsa_circ_0014235 acts as a miRNA sponge for miR-520a-5p to relieve repression of CDK4 (ceRNA axis).
Biological pathway or process:

chemoresistance (promotes); proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model

Clinical significance:

Proposed as a promising biomarker for NSCLC (including via serum-derived exosomes) and for NSCLC treatment.

Description:

hsa_circ_0014235 is up-regulated in NSCLC tumor tissues, NSCLC cells, and NSCLC patient serum-derived exosomes. It promotes cisplatin (DDP) chemoresistance and malignant phenotypes (proliferation, migration/invasion, cell-cycle progression) while inhibiting apoptosis, partly through an exosome-associated ceRNA axis hsa_circ_0014235/miR-520a-5p/CDK4.

Confidence score:

0.6833

Other information
Title:

Exosome-transferred hsa_circ_0014235 promotes DDP chemoresistance and deteriorates the development of non-small cell lung cancer by mediating the miR-520a-5p/CDK4 pathway.

Journal: Cancer cell international
Published: 2020
PubMed ID: 33292236
Study type:

combined biological and clinical study

Data availability: The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
Code availability: -