circRNA basic information
circBase ID: hsa_circ_0005218
Name: hsa_circ_FAM120A
Synonym: circ_018131
Host Gene: -
Genomic location(hg19): chr9:96233422-96259881:+
Genomic location(hg38): chr9:93471140-93497599:+
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; adjacent liver specimens; adjacent non-tumorous tissues; para-cancerous tissues
Cell lines:

MHCC-97H; SMMC-7721; Hep3B; Huh7; HepG2; SK-HEP-1; HL-7702
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CDK1
Associated microRNA: miR-31-5p
Biological function: Promotes HCC cell proliferation, migration, invasion, metastasis, cell cycle progression and tumorigenesis; associated with HCC early recurrence and poor prognosis.
Molecular mechanism: circ_0005218 acts as a miR-31-5p sponge to weaken miR-31-5p-mediated suppression of CDK1, thereby promoting HCC cell invasion, migration and tumorigenesis.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); cell cycle (promotes)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circ_0005218 expression is associated with tumor number, microvascular invasion, tumor early recurrence, poor OS and DFS, and may serve as a biomarker for HCC early recurrence prediction and a therapeutic target.
Description:

hsa_circ_0005218/circ_0005218 is up-regulated in HCC, especially early recurrent HCC tissues, and high expression predicts poor OS and DFS. Functionally, it promotes HCC proliferation, migration, invasion, metastasis, cell cycle progression and xenograft growth. Mechanistically, it sponges miR-31-5p to relieve suppression of CDK1, supporting a circ_0005218/miR-31-5p/CDK1 regulatory axis and potential use as an early recurrence biomarker and therapeutic target.
Confidence score:

0.8793
Other information
Title:

Circular RNA hsa_circ_0005218 promotes the early recurrence of hepatocellular carcinoma by targeting the miR-31-5p/CDK1 pathway.

Journal: Heliyon
Published: 2023
PubMed ID: 37035389
Study type:

combined biological and clinical study
Data availability: Data will be made available on request; Supplementary data to this article can be found online at https://doi.org/10.1016/j.heliyon.2023.e14816
Code availability: -