| Expression pattern: |
DN |
| Associated gene: |
PTEN, AKT, CDK2, PTEN mRNA 3'UTR |
| Associated microRNA: |
hsa-miR-136-5p |
| Biological function: |
hsa_circ_0006282 acts as a tumor suppressor in gastric cancer; its knockdown promotes cell proliferation, growth ability, migration and resistance to apoptosis, whereas overexpression inhibits these malignant behaviors. |
| Molecular mechanism: |
hsa_circ_0006282 competitively binds hsa-miR-136-5p as a miRNA sponge, induces PTEN expression, and modulates the PTEN/AKT signaling pathway. |
| Biological pathway or process: |
PI3K/AKT (inhibits); proliferation (inhibits); migration (inhibits); apoptosis (promotes); cell cycle (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0006282 may serve as a potential diagnostic biomarker for gastric cancer, with an AUC of 0.655, cutoff value of 17.47, sensitivity of 47.3%, and specificity of 80.6%. |
| Description: |
hsa_circ_0006282 is down-regulated in gastric cancer tissues and cells and functions as a tumor suppressor. It suppresses gastric cancer cell proliferation, migration and resistance to apoptosis by sponging hsa-miR-136-5p, promoting PTEN expression, and modulating the PTEN/AKT pathway. The study also suggests potential diagnostic biomarker value for gastric cancer. |
| Confidence score: |
0.7992 |