| Expression pattern: |
DN |
| Associated gene: |
TRAF3 |
| Associated microRNA: |
miRNA-95-3p |
| Biological function: |
inhibits cell proliferation/growth and promotes apoptosis in cholangiocarcinoma cells |
| Molecular mechanism: |
acts as a sponge for miRNA-95-3p and thereby modulates the downstream target TRAF3 (validated by dual-luciferase reporter assays) to suppress cholangiocarcinoma progression |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
may become a novel approach for treating CHOL / served as a novo-biomarker for CHOL |
| Description: |
CircRNA SMARCA5 is down-regulated in cholangiocarcinoma tissues and cell lines. Its overexpression suppresses proliferation and increases apoptosis, mechanistically by binding miRNA-95-3p and relieving miRNA-95-3p-mediated repression of TRAF3 (circRNA SMARCA5/miRNA-95-3p/TRAF3 axis). |
| Confidence score: |
0.6479 |