circRNA basic information
circBase ID: hsa_circ_0067934
Name: hsa_circ_PRKCI
Synonym: circPRKCI
Host Gene: PRKCI
Genomic location(hg19): chr3:170013698-170015181:+
Genomic location(hg38): chr3:170295910-170297393:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005061
MONDO name: lung adenocarcinoma
Disease details: lung adenocarcinoma / LUAD
Disease DO ID:
 3910
Disease MeSH ID:
 C538231
Disease NCIt ID:
 C3512
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary LAC tissues; adjacent non-tumor tissues; lung cancer tissues; LAC tissue
Cell lines:

A549; NCI-H1975; NCI-H1703; NCI-H226; NCI-H46; PC9; NCI-H1299; SPC-A1; HCC827; HBE; K562
In vivo animal model:

cell line-derived xenograft; patient-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: E2F7, Ago2, p21, Cyclin D1
Associated microRNA: miR-545, miR-589
Biological function: Promotes proliferation, migration, invasion and tumorigenesis of LAC cells; supports cell cycle progression; may reduce sensitivity to gefitinib inhibition.
Molecular mechanism: circPRKCI is overexpressed partly due to PRKCI/3q26.2 amplification and acts through a ceRNA mechanism by sponging miR-545 and miR-589, relieving suppression of E2F7 and altering p21/Cyclin D1 signaling.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); ceRNA regulation (promotes); tumorigenesis (promotes); drug resistance (promotes)
Detected method:
Q
H
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; ISH (In Situ Hybridization); FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; TUNEL; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circPRKCI expression correlates with larger tumor size, higher TNM stage and shorter overall survival; circPRKCI may serve as a potential biomarker and therapeutic target for LAC.
Description:

circPRKCI is a PRKCI-derived circular RNA from the 3q26.2 amplicon that is up-regulated in lung adenocarcinoma. It promotes LAC cell proliferation, invasion/migration and tumor growth by sponging miR-545 and miR-589 to increase E2F7 and regulate downstream p21/Cyclin D1 signaling. High circPRKCI expression is associated with larger tumors, advanced TNM stage and poor overall survival, and targeting circPRKCI suppresses xenograft growth and enhances gefitinib inhibition in EGFR-mutant cells.
Confidence score:

0.8855
Other information
Title:

The Circular RNA circPRKCI Promotes Tumor Growth in Lung Adenocarcinoma.

Journal: Cancer research
Published: 2018
PubMed ID: 29588350
Study type:

combined biological and clinical study
Data availability: GSE101586
Code availability: -