circRNA basic information
circBase ID: hsa_circ_0002137
Name: -
Synonym: circ_0002137
Host Gene: -
Genomic location(hg19): chr1:224918163-224922408:+
Genomic location(hg38): chr1:224730461-224734706:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

osteosarcoma tumours; paracancerous tissue; surrounding non-tumour tissue
Cell lines:

MG-63; U2OS; 143B; G292; hFOB 1.19; SJSA1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: IGF1R
Associated microRNA: miR-433-3p
Biological function: Silencing circ_0002137 suppresses osteosarcoma cell proliferation, invasion and migration, induces G0/G1 arrest and promotes apoptosis; circ_0002137 knockdown suppresses tumor growth in vivo.
Molecular mechanism: circ_0002137 acts as a sponge for miR-433-3p and thereby regulates IGF1R expression (ceRNA-like mechanism), affecting osteosarcoma progression.
Biological pathway or process:

proliferation (inhibits); apoptosis (promotes); cell cycle (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Clinical Sample Validation; Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; RNA Pull-Down; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model
Clinical significance:

-
Description:

hsa_circ_0002137 is up-regulated in osteosarcoma tissues and OS cell lines. Functionally, circ_0002137 knockdown inhibits proliferation, invasion and migration, induces G0/G1 arrest, and promotes apoptosis, and suppresses xenograft tumor growth. Mechanistically, circ_0002137 binds (sponges) miR-433-3p to regulate IGF1R expression, forming a circ_0002137/miR-433-3p/IGF1R axis in OS.
Confidence score:

0.7418
Other information
Title:

Circular RNA circ_0002137 regulated the progression of osteosarcoma through regulating miR-433-3p/ IGF1R axis.

Journal: Journal of cellular and molecular medicine
Published: 2022
PubMed ID: 33621401
Study type:

combined biological and clinical study
Data availability: available from the corresponding author upon request
Code availability: -