| Expression pattern: |
UP |
| Associated gene: |
RAB3D |
| Associated microRNA: |
miR-877-5p |
| Biological function: |
promotes proliferation, migration and invasion and inhibits apoptosis in NSCLC; promotes tumor formation in vivo |
| Molecular mechanism: |
ceRNA mechanism: circPRH1-PRR4 sponges miR-877-5p to upregulate RAB3D |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Clinical Sample Validation; Survival Analysis; Transfection; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
NSCLC patients with high expression of circPRH1-PRR4 had a poor prognosis; higher in high-metastatic NSCLC tissues than in low-metastatic NSCLC tissues |
| Description: |
circPRH1-PRR4 is up-regulated in NSCLC tissues and cell lines and functions as an oncogenic circRNA. It sponges miR-877-5p to increase RAB3D expression, thereby promoting proliferation/migration/invasion and suppressing apoptosis, and its knockdown reduces tumor growth in a xenograft model. |
| Confidence score: |
0.7735 |