| Expression pattern: |
UP |
| Associated gene: |
CDK6 |
| Associated microRNA: |
miR-29a, miR-29b, miR-29c, miR-299, miR-29b-3p, miR-29c-3p, miR-29a-3p |
| Biological function: |
sustains cell cycle progression and promotes proliferation; knockdown inhibits proliferation and induces G1/S arrest; promotes tumor growth in vivo |
| Molecular mechanism: |
Acts as a competing endogenous RNA (ceRNA) that sponges miR-29 family members to regulate CDK6 expression |
| Biological pathway or process: |
ceRNA regulation (promotes); cell cycle (promotes); proliferation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; RIP (RNA Immunoprecipitation); MTT; BrdU; Cell Cycle Assay; In Vivo Animal Model; IF (Immunofluorescence); Western Blot; IHC (Immunohistochemistry) |
| Clinical significance: |
Potential target for OSCC therapy |
| Description: |
circRNA_100290 is up-regulated in OSCC tissues/cell lines and promotes OSCC cell-cycle progression and proliferation as well as tumor growth in vivo. Mechanistically, it acts as a ceRNA/miRNA sponge for the miR-29 family (miR-29a/b/c), thereby relieving miR-29-mediated repression of CDK6 and sustaining CDK6 expression. |
| Confidence score: |
0.8174 |