| Expression pattern: |
UN |
| Associated gene: |
Cyclin D1 |
| Associated microRNA: |
hsa-miR-4768-5p |
| Biological function: |
Silencing of circANKRD12 increases invasion and migration, decreases proliferation, induces G0/G1 arrest, and decreases oxidative phosphorylation in cancer cells. |
| Molecular mechanism: |
circANKRD12 may act as a competing endogenous RNA (ceRNA) to sponge microRNAs shared with Cyclin D1, thereby regulating cyclin D1 and cell-cycle-related phenotypic switching; pathway-level changes include interferon signaling and cell cycle checkpoint regulation. |
| Biological pathway or process: |
cell cycle (inhibits); proliferation (inhibits); migration (promotes); invasion (promotes); oxidative phosphorylation (inhibits); AMPK (inhibits); JAK/STAT (promotes); NF-kappaB (promotes); inflammation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Wound Healing Assay; Transwell Assay; CCK8; Cell Cycle Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
has the potential to become a new clinical biomarker |
| Description: |
circANKRD12 is a cytoplasmic, RNase R-resistant circRNA derived from ANKRD12 that is abundant across multiple cancer cell lines. siRNA knockdown of circANKRD12 drives a phenotypic switch featuring reduced proliferation and G0/G1 arrest but increased migration/invasion, with decreased oxidative phosphorylation. The study suggests a potential ceRNA mechanism involving shared miRNA binding with Cyclin D1 (e.g., hsa-miR-4768-5p) and proposes biomarker potential. |
| Confidence score: |
0.7011 |