| Expression pattern: |
UP |
| Associated gene: |
iNOS, TNFalpha, eIF4A3 |
| Associated microRNA: |
miR-939 |
| Biological function: |
promotes macrophage proliferation; promotes M1 macrophage polarization and pro-inflammatory cytokine secretion; suppresses M2 polarization |
| Molecular mechanism: |
ceRNA sponging of miR-939; regulated by RBP eIF4A3 binding to flanking sequences; activation of NF-kappaB and inhibition of Akt/mTOR in macrophage polarization context |
| Biological pathway or process: |
NF-kappaB (promotes); PI3K/AKT/mTOR (inhibits); proliferation (promotes); macrophage polarization (other); inflammation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; EdU Staining; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; ELISA; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
circIFNGR2 expression in PBMCs was positively correlated with ASDASCRP score, BASDAI score, and serum C-reactive protein levels (disease activity). |
| Description: |
circIFNGR2 is up-regulated in PBMCs from ankylosing spondylitis patients and correlates with disease activity. It promotes macrophage proliferation and pro-inflammatory M1 polarization while inhibiting M2 polarization by sponging miR-939, thereby enhancing iNOS/TNFalpha expression and modulating NF-kappaB and Akt/mTOR signaling. Its biogenesis is facilitated by the RBP eIF4A3 binding to flanking sequences. |
| Confidence score: |
0.7951 |