| Expression pattern: |
UP |
| Associated gene: |
STAT3, AR |
| Associated microRNA: |
miR-665, miR-671-5p |
| Biological function: |
inhibits TNBC cell proliferation |
| Molecular mechanism: |
circAR-E2E4 sponges miR-665 (and miR-671-5p) and positively regulates STAT3; circAR-E2E4 is regulated by AR. |
| Biological pathway or process: |
proliferation (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA Pull-Down; Transfection; CCK8; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circAR-E2E4 has the potential to be a promising diagnostic, prognostic, and therapeutic target in TNBC |
| Description: |
circAR-E2E4 (from the AR gene) promotes TNBC cell proliferation and is positively regulated by AR. Mechanistically, circAR-E2E4 acts as a miRNA sponge (miR-665/miR-671-5p), and circAR-E2E4 knockdown decreases STAT3 mRNA/protein, supporting a circAR-E2E4-miR-665-STAT3 regulatory axis in TNBC. |
| Confidence score: |
0.7164 |