| Expression pattern: |
DN |
| Associated gene: |
PES1, Claudin-2 |
| Associated microRNA: |
- |
| Biological function: |
Inhibits colonic epithelial cell proliferation; antagonizes barrier function (loss enhances barrier integrity). |
| Molecular mechanism: |
Circular CDKN2B-AS1 is produced by back-splicing; down-regulation of CDKN2B-AS1 reduces Claudin-2 expression and increases TEER (barrier integrity). |
| Biological pathway or process: |
proliferation (inhibits); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; ISH (In Situ Hybridization); Clinical Sample Validation; Transfection; CCK8; Bioinformatics Analysis |
| Clinical significance: |
Decreased circular CDKN2B-AS1 in UC-active patient biopsies compared to controls. |
| Description: |
The circular form of CDKN2B-AS1 is down-regulated in UC-active/IBD colonic biopsies. Experimental knockdown (targeting both linear and circular transcripts) in Caco2 BBE cells increases proliferation and enhances epithelial barrier function, associated with reduced Claudin-2 levels, and the RNA is primarily nuclear. |
| Confidence score: |
0.7091 |