| Expression pattern: |
DN |
| Associated gene: |
Bcl-2, procaspase-3 |
| Associated microRNA: |
miR-659 |
| Biological function: |
Suppresses breast cancer progression by inhibiting proliferation/growth, colony formation, invasion and migration, reducing tumor formation in vivo, and promoting intrinsic apoptosis. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-659; miR-659 mimic reverses the tumor-suppressing and apoptosis-inducing effects of hsa_circ_0068033, and binding is supported by luciferase reporter assay. |
| Biological pathway or process: |
proliferation (inhibits); invasion (inhibits); migration (inhibits); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; ISH (In Situ Hybridization); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; TUNEL; Western Blot; Luciferase Reporter Assay; In Vivo Animal Model; ROC Analysis |
| Clinical significance: |
Downregulated in BC tissues and correlated with tumor size and TNM stage; ROC analysis indicates diagnostic value (AUC=0.8480) for distinguishing BC from non-tumor controls. |
| Description: |
hsa_circ_0068033 is down-regulated in breast cancer and functions as a tumor suppressor. Its overexpression inhibits proliferation/colony formation, migration and invasion, reduces xenograft tumorigenicity, and promotes intrinsic apoptosis, at least partly by sponging miR-659, supporting diagnostic utility (AUC=0.8480). |
| Confidence score: |
0.8126 |