| Expression pattern: |
UP |
| Associated gene: |
PTEN mRNA, PTEN, MMP13, MMP-13, NF-KB, NF-KBp65, LPB |
| Associated microRNA: |
- |
| Biological function: |
circZNF652 promotes LPS-induced inflammation and positively regulates LPS-induced apoptosis of chondrocytes; circZNF652 may promote OA progression by increasing PTEN, MMP-13, and NF-KB expression. |
| Molecular mechanism: |
circZNF652 upregulates PTEN expression at mRNA and protein levels, affects MMP-13 and NF-KB expression, and thereby promotes LPS-induced apoptosis of chondrocytes in OA. |
| Biological pathway or process: |
apoptosis (promotes); NF-kappaB (promotes); inflammation (promotes); PI3K/AKT (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; Western Blot; Annexin V/PI Flow Cytometry; ELISA |
| Clinical significance: |
circZNF652 was overexpressed in synovial fluid samples from OA patients compared with healthy controls and circZNF652 silencing might serve as a potential therapeutic target for OA. |
| Description: |
circZNF652 is overexpressed in synovial fluid from osteoarthritis patients and is induced by LPS in OA chondrocytes. Functionally, circZNF652 promotes LPS-induced chondrocyte apoptosis and may accelerate OA progression by upregulating PTEN and affecting MMP-13 and NF-KB expression. The study suggests circZNF652 silencing as a potential therapeutic strategy for OA. |
| Confidence score: |
0.5358 |