| Expression pattern: |
UP |
| Associated gene: |
TCF4 |
| Associated microRNA: |
miR-450b-5p |
| Biological function: |
circ_0004662 promotes LPS-induced OA chondrocyte injury, apoptosis and inflammatory cytokine secretion; knockdown improves cell viability and attenuates inflammation and apoptosis. |
| Molecular mechanism: |
circ_0004662 functions as a ceRNA sponge for miR-450b-5p, thereby derepressing/upregulating TCF4 expression in OA chondrocytes. |
| Biological pathway or process: |
apoptosis (promotes); inflammation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model |
| Clinical significance: |
circ_0004662 has potential diagnostic biomarker value for OA; ROC AUC was 0.9652. |
| Description: |
circ_0004662 is upregulated in OA cartilage tissues and LPS-stimulated human chondrocytes. It promotes inflammatory chondrocyte injury by acting as a sponge for miR-450b-5p and increasing TCF4 expression; its knockdown improves cell viability and reduces apoptosis and inflammatory cytokine secretion. The study also suggests circ_0004662 may have diagnostic biomarker potential for OA. |
| Confidence score: |
0.7756 |