circRNA basic information
circBase ID: hsa_circ_0097010
Name: hsa_circ_SLC5A8
Synonym: circ_0097010
Host Gene: SLC5A8
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005076
MONDO name: periodontitis
Disease details: periodontitis
Disease DO ID:
824, 9893
Disease MeSH ID:
D010518
Disease NCIt ID:
C34918
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

gingival tissue

Cell lines:

hPDLCs

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: KLF6, Ago2
Associated microRNA: miR-769-5p
Biological function: Downregulation of circ_0097010 alleviates LPS-induced apoptosis and inflammation in hPDLCs and rescues LPS-imposed viability and proliferation defects.
Molecular mechanism: circ_0097010 functions as a ceRNA by sponging miR-769-5p, thereby regulating KLF6 expression; validated via luciferase reporter, Ago2-RIP, and RNA pull-down assays.
Biological pathway or process:

apoptosis (inhibits); inflammation (inhibits); proliferation (promotes); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; ELISA; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Clinical Sample Validation; Bioinformatics Analysis

Clinical significance:

circ_0097010 expression is increased in periodontitis gingival samples and is progressively upregulated with increasing severity; suggested potential diagnostic value of the circ_0097010/miR-769-5p/KLF6 cascade.

Description:

circ_0097010 is upregulated in periodontitis gingival tissues and in LPS-stimulated hPDLCs. Silencing circ_0097010 reduces LPS-induced apoptosis and inflammatory/oxidative injury by acting as a sponge for miR-769-5p, thereby decreasing KLF6 expression (circ_0097010/miR-769-5p/KLF6 ceRNA axis).

Confidence score:

0.779

Other information
Title:

Anti-apoptosis and anti-inflammation activity of circ_0097010 downregulation in lipopolysaccharide-stimulated periodontal ligament cells by miR-769-5p/Krüppel like factor 6 axis.

Journal: Journal of dental sciences
Published: 2023
PubMed ID: 36643256
Study type:

combined biological and clinical study

Data availability: Supplementary data to this article can be found online at https://doi.org/10.1016/j.jds.2022.04.024.
Code availability: -