| Expression pattern: |
UP |
| Associated gene: |
PTBP1, FASN, AGO2 |
| Associated microRNA: |
- |
| Biological function: |
Promotes ICC cell proliferation, colony formation, tumor growth and metastasis; inhibits apoptosis and G0/G1 arrest; promotes lipid metabolism reprogramming (especially unsaturated fatty acids) and reduces oxidative stress/ROS and lipid peroxidation. |
| Molecular mechanism: |
Binds PTBP1 and protects it from ubiquitin/proteasome-dependent degradation, thereby promoting PTBP1-mediated cytoplasmic export of FASN mRNA to increase FASN translation; regulates lipid metabolic profile (unsaturated fatty acids) and redox homeostasis. |
| Biological pathway or process: |
lipid metabolism (promotes); oxidative phosphorylation (other); ferroptosis (inhibits); NF-kappaB (inhibits); apoptosis (inhibits); cell cycle (promotes); proliferation (promotes); metastasis (promotes); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; RNA Pull-Down; RIP (RNA Immunoprecipitation); Bioinformatics Analysis; Flow Cytometry（Non-apoptosis/cycle） |
| Clinical significance: |
Frequently upregulated in ICC tissues, positively correlated with malignant features and tumor size, and higher expression was slightly correlated with shorter OS / predicts poor survival. |
| Description: |
circMBOAT2 (hsa_circ_0007334) is upregulated in intrahepatic cholangiocarcinoma and promotes tumor growth and metastatic phenotypes. It acts mainly by binding and stabilizing PTBP1 (reducing PTBP1 ubiquitin/proteasome degradation), thereby enhancing PTBP1-mediated cytoplasmic export of FASN mRNA and increasing FASN translation. This reprograms lipid metabolism toward unsaturated fatty acids and helps maintain redox homeostasis (lower ROS/lipid peroxidation), contributing to ICC progression and suggesting a potential therapeutic target. |
| Confidence score: |
0.8661 |