circRNA basic information
circBase ID: hsa_circ_0000004
Name: -
Synonym: circ_0000004
Host Gene: -
Genomic location(hg19): chr1:1430864-1464711:+
Genomic location(hg38): chr1:1495484-1529331:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

human osteosarcoma tissue; normal bone tissue
Cell lines:

143B; MG-63
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PLOD2, APLN
Associated microRNA: miR-1303
Biological function: promotes osteosarcoma cell migration and metastasis; increases PLOD2 expression
Molecular mechanism: hsa_circ_0000004 acts as a miR-1303 sponge to relieve miR-1303 repression on PLOD2, thereby increasing PLOD2 and promoting migration
Biological pathway or process:

migration (promotes); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Luciferase Reporter Assay; RNA Pull-Down; Transfection; Transwell Assay; Western Blot; Clinical Sample Validation
Clinical significance:

-
Description:

In osteosarcoma, hsa_circ_0000004 is upregulated and promotes cell migration/metastatic behavior by sponging miR-1303, thereby increasing PLOD2 expression. It functions as part of an APLN-driven ceRNA axis (hsa_circ_0000004/miR-1303/PLOD2) linked to Hippo pathway activation.
Confidence score:

0.6672
Other information
Title:

Apelin promotes osteosarcoma metastasis by upregulating PLOD2 expression via the Hippo signaling pathway and hsa_circ_0000004/miR-1303 axis.

Journal: International journal of biological sciences
Published: 2023
PubMed ID: 36632453
Study type:

combined biological and clinical study
Data availability: GSE16088; GSE12865
Code availability: -