| Expression pattern: |
UP |
| Associated gene: |
MMP1, MMP2, MMP9, Ago2 |
| Associated microRNA: |
miR-1238 |
| Biological function: |
promotes CRC growth, proliferation, invasion and metastasis |
| Molecular mechanism: |
circMMP1 acts as a ceRNA sponge for miR-1238, relieving repression of MMP1/MMP2/MMP9 and increasing their expression; the interaction is supported by luciferase reporter and AGO2-RIP assays. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining |
| Clinical significance: |
Potential diagnostic biomarker for early CRC screening; potential prognostic implications |
| Description: |
circMMP1 (hsa_circ_0024109) is up-regulated in colorectal cancer tissues and cell lines and mainly localizes in the cytoplasm. Functionally, circMMP1 promotes CRC proliferation, invasion and metastasis. Mechanistically, it sponges miR-1238 to relieve repression of MMP1/MMP2/MMP9, increasing their expression and driving CRC progression. |
| Confidence score: |
0.84 |