| Expression pattern: |
DN |
| Associated gene: |
KLF6 |
| Associated microRNA: |
miR-653 |
| Biological function: |
circSETD3 overexpression blocks the promoting effects of miR-653 on CRC cell proliferation, invasion and migration and affects apoptosis via sponging miR-653. |
| Molecular mechanism: |
ceRNA sponge mechanism: circSETD3 sponges miR-653, thereby regulating KLF6. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; RT-qPCR; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Transwell Assay |
| Clinical significance: |
circSETD3/miR-653/KLF6 axis may be an effective therapeutic target for CRC patients. |
| Description: |
In CRC cells, circSETD3 (hsa_circ_0000567) is down-regulated and acts as a miRNA sponge for miR-653, thereby relieving miR-653-mediated suppression of KLF6. circSETD3 overexpression counteracts miR-653-driven proliferation, migration and invasion and supports apoptosis, suggesting a tumor-suppressive role via the circSETD3/miR-653/KLF6 axis. |
| Confidence score: |
0.6952 |