circRNA basic information
circBase ID: hsa_circ_0082002
Name: hsa_circ_MET
Synonym: circMET
Host Gene: MET
Genomic location(hg19): chr7:116339124-116340338:+
Genomic location(hg38): chr7:116699070-116700284:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0018177
MONDO name: glioblastoma
Disease details: glioblastoma / GBM
Disease DO ID:
 3068
Disease MeSH ID:
 D005909
Disease NCIt ID:
 C3058
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioma tissue; adjacent normal brain tissues; cerebrospinal fluid
Cell lines:

GSC387; GSC456; GSC28; GSC23; 293T; U118; U251; SNB19; NHA
In vivo animal model:

cell line-derived xenograft; genetically engineered animal model; other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: YTHDF2
Associated microRNA: -
Biological function: promotes glioblastoma tumorigenesis and stem cell malignancy by activating MET signaling
Molecular mechanism: circMET is an m6A-modified coding circRNA; YTHDF2 facilitates its translation into MET404, which activates MET signaling independent of HGF
Biological pathway or process:

ceRNA regulation (not specified); other pathway/process (promotes)
Detected method:
Q
B
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA-seq; Bioinformatics Analysis; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; ELISA; Co-IP; RNA Pull-Down
Clinical significance:

High MET404 expression predicted worse overall survival of GBM patients
Description:

This study identifies circMET as an m6A-modified circular RNA from the MET gene that is highly expressed in GBM/GSCs. circMET interacts with the m6A reader YTHDF2 to facilitate translation of the MET404 protein, which drives GBM tumorigenesis by constitutively activating MET signaling independent of HGF.
Confidence score:

0.8886
Other information
Title:

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis.

Journal: Nature communications
Published: 2023
PubMed ID: 37491377
Study type:

combined biological and clinical study
Data availability: HRA002365; PRJNA973050
Code availability: -