circRNA basic information
circBase ID: hsa_circ_0058122
Name: hsa_circ_FN1
Synonym: circFN1
Host Gene: FN1
Genomic location(hg19): chr2:216269111-216285524:-
Genomic location(hg38): chr2:215404388-215420801:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0012126
MONDO name: familial avascular necrosis of femoral head
Disease details: steroid-induced osteonecrosis of the femoral head / SONFH
Disease DO ID:
 -
Disease MeSH ID:
 D005271
Disease NCIt ID:
 -
Disease ICD11 ID:
 1216860745
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

femoral head tissues; SONFH tissues; adjacent normal tissues
Cell lines:

HUVECs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: IGFBP5
Associated microRNA: miR-7974
Biological function: promotes DEX-induced apoptosis of HUVECs
Molecular mechanism: Acts as a miRNA sponge: hsa_circ_0058122 binds miR-7974 to relieve repression of IGFBP5, increasing IGFBP5 and promoting apoptosis in DEX-treated HUVECs.
Biological pathway or process:

apoptosis (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; divergent primers PCR; RNase R Treatment; FISH / smFISH; Luciferase Reporter Assay; Transfection; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis; Clinical Sample Validation
Clinical significance:

may serve as a potential diagnostic biomarker or a drug target for SONFH therapy
Description:

hsa_circ_0058122 (circFN1), derived from FN1, is up-regulated in SONFH femoral head tissues and in DEX-treated HUVECs. It promotes DEX-induced endothelial apoptosis by sponging miR-7974 and upregulating IGFBP5 (hsa_circ_0058122/miR-7974/IGFBP5 axis).
Confidence score:

0.7791
Other information
Title:

hsa_circ_0058122 knockdown prevents steroid-induced osteonecrosis of the femoral head by inhibiting human umbilical vein endothelial cells apoptosis via the miR-7974/IGFBP5 axis.

Journal: Journal of clinical laboratory analysis
Published: 2022
PubMed ID: 35274778
Study type:

combined biological and clinical study
Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Code availability: -