| Expression pattern: |
DN |
| Associated gene: |
MG53, AGO2 |
| Associated microRNA: |
miR-497-5p |
| Biological function: |
MSCs-derived exosomal circRTN4 alleviates sepsis-induced myocardial injury, suppresses cardiomyocyte apoptosis, reduces ROS accumulation and inflammatory cytokine levels, and improves cardiomyocyte survival. |
| Molecular mechanism: |
MSCs-derived exosomal circRTN4 acts as a miR-497-5p sponge to upregulate MG53 expression, thereby relieving LPS-stimulated cardiomyocyte damage and sepsis-induced myocardial injury. |
| Biological pathway or process: |
apoptosis (inhibits); proliferation (promotes); inflammation (inhibits); ceRNA regulation (other); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Transfection; MTT; CCK8; Annexin V/PI Flow Cytometry; TUNEL; H&E Staining; ELISA; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
MSCs-derived exosomal circRTN4 may be applied as a promising therapeutic approach for sepsis-induced myocardial injury. |
| Description: |
This study found that circRTN4 is down-regulated in CLP rat cardiac tissues and LPS-treated cardiomyocytes. MSC-derived exosomal circRTN4 protects against sepsis-induced myocardial injury by reducing apoptosis, oxidative stress, and inflammatory cytokines. Mechanistically, circRTN4 functions as a miR-497-5p sponge to increase MG53 expression through the circRTN4/miR-497-5p/MG53 axis. |
| Confidence score: |
0.7827 |