circRNA basic information
circBase ID: hsa_circ_0006220
Name: hsa_circ_TADA2A
Synonym: circ_0006220
Host Gene: TADA2A
Genomic location(hg19): chr17:35800605-35800763:+
Genomic location(hg38): chr17:37440504-37440662:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent normal lung tissues; serum

Cell lines:

A-549; NCI-H1299; BEAS-2B; HUVEC; 293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: GOT2
Associated microRNA: miR-342-3p
Biological function: promotes proliferation, migration, invasion, and angiogenesis; inhibits apoptosis in NSCLC cells
Molecular mechanism: Acts as a miRNA sponge (ceRNA) for miR-342-3p to up-regulate GOT2.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); ceRNA regulation (promotes)

Detected method:
Q
M
Validation methods:

RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Transfection; CCK8; Colony Formation Assay; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis

Clinical significance:

Circ_0006220 was upregulated in serum samples of NSCLC patients and high circ_0006220 expression was correlated with advanced TNM stages, distant metastasis, and shorter survival time.

Description:

Circ_0006220 is up-regulated in NSCLC tissues, cell lines, and patient serum, and higher levels associate with advanced disease and worse survival. Functionally, it promotes NSCLC malignant phenotypes by acting as a ceRNA that sponges miR-342-3p and thereby increases GOT2 expression, enhancing proliferation, migration/invasion, and angiogenesis while suppressing apoptosis.

Confidence score:

0.8498

Other information
Title:

Circ_0006220 Contributes to NSCLC Progression through miR-342-3p/GOT2 Axis.

Journal: Annals of thoracic and cardiovascular surgery : official journal of the Association of Thoracic and Cardiovascular Surgeons of Asia
Published: 2023
PubMed ID: 36575008
Study type:

combined biological and clinical study

Data availability: GSE101586
Code availability: -