| Expression pattern: |
DN |
| Associated gene: |
PTPRD, beta-catenin, wnt1 |
| Associated microRNA: |
miR-324-5p |
| Biological function: |
inhibits proliferation, migration, invasion, and epithelial-mesenchymal transition; suppresses tumor growth in vivo |
| Molecular mechanism: |
ceRNA/miRNA sponge mechanism via binding miR-324-5p; associated with increased PTPRD and inhibition of beta-catenin and wnt1 |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); Wnt/beta-catenin (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Sanger Sequencing; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; Clinical Sample Validation; ROC Analysis; Survival Analysis; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
AUC 0.838 with sensitivity 82.3% and specificity 77.2% for distinguishing GC tissues; no significant difference in overall survival between low and high expression groups |
| Description: |
circ_0049447 (derived from RAB3D exons) is down-regulated in gastric cancer and acts as a tumor suppressor by inhibiting proliferation, migration, invasion, and EMT in GC cells and xenografts. Mechanistically, it binds (sponges) miR-324-5p (validated by luciferase), with associated upregulation of PTPRD and inhibition of beta-catenin/wnt1 signals. It shows diagnostic potential (ROC AUC 0.838) but no significant association with overall survival in this cohort. |
| Confidence score: |
0.7831 |