circRNA basic information
circBase ID: hsa_circ_0020378
Name: hsa_circ_DOCK1
Synonym: circDOCK1
Host Gene: DOCK1
Genomic location(hg19): chr10:128594022-128926028:+
Genomic location(hg38): chr10:126905453-127127764:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OS tissues; healthy paracarcinoma controls; tumor samples
Cell lines:

hFOB 1.19; HOS; U2OS
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: DNMT3A
Associated microRNA: miR-186
Biological function: Promotes osteosarcoma progression by enhancing cell proliferation, motility, invasion, and xenograft tumor growth.
Molecular mechanism: Acts as a miRNA sponge for miR-186 to upregulate DNMT3A expression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (other); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; Clinical Sample Validation
Clinical significance:

-
Description:

circDOCK1 is up-regulated in osteosarcoma tissues and cell lines and promotes proliferation, migration/motility, invasion, and xenograft tumor growth. Mechanistically, circDOCK1 sponges miR-186 to relieve repression of DNMT3A, forming a circDOCK1/miR-186/DNMT3A ceRNA axis.
Confidence score:

0.869
Other information
Title:

CircDOCK1 Regulates miR-186/DNMT3A to Promote Osteosarcoma Progression.

Journal: Biomedicines
Published: 2022
PubMed ID: 36551768
Study type:

combined biological and clinical study
Data availability: GSE140256
Code availability: -