circRNA basic information
circBase ID: hsa_circ_0000994
Name: hsa_circ_SLC8A1
Synonym: CircSLC8A1
Host Gene: SLC8A1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005381
MONDO name: bone disorder
Disease details: periprosthetic osteolysis / PPOL
Disease DO ID:
 0080001
Disease MeSH ID:
 D001847
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

periprosthetic tissues
Cell lines:

hBMSCs; HEK-293T
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
DN
Associated gene: RUNX1
Associated microRNA: miR-144-3p
Biological function: promotes osteogenic differentiation and inhibits apoptosis in hBMSCs, alleviating PPOL
Molecular mechanism: ceRNA mechanism: CircSLC8A1 sponges miR-144-3p to regulate RUNX1
Biological pathway or process:

osteogenesis (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; FISH / smFISH; Transfection; Annexin V/PI Flow Cytometry; IF (Immunofluorescence); Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

-
Description:

CircSLC8A1 is down-regulated in PPOL periprosthetic tissues and in Ti particle-induced PPOL cell models. Functionally, it promotes osteogenic differentiation and suppresses apoptosis in hBMSCs. Mechanistically, it acts as a ceRNA by sponging miR-144-3p to derepress RUNX1 (CircSLC8A1/miR-144-3p/RUNX1), thereby alleviating PPOL in vivo.
Confidence score:

0.8203
Other information
Title:

Circular RNA CircSLC8A1 contributes to osteogenic differentiation in hBMSCs via CircSLC8A1/miR-144-3p/RUNX1 in periprosthetic osteolysis.

Journal: Journal of cellular and molecular medicine
Published: 2023
PubMed ID: 36541023
Study type:

combined biological and clinical study
Data availability: available from the corresponding author upon reasonable request
Code availability: -