| Expression pattern: |
UP |
| Associated gene: |
IGF2BP3, SLC7A5 mRNA |
| Associated microRNA: |
- |
| Biological function: |
promotes gastric cancer cell proliferation/growth in vitro and in vivo |
| Molecular mechanism: |
acts as a scaffold to facilitate IGF2BP3-SLC7A5 mRNA interaction, forms a circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex, enhances SLC7A5 mRNA stability, and activates AKT/mTOR signaling |
| Biological pathway or process: |
proliferation (promotes); PI3K/AKT/mTOR (promotes); mRNA stability (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RIP (RNA Immunoprecipitation); RNA-seq; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; ISH (In Situ Hybridization); RT-qPCR; RNA Pull-Down; IF (Immunofluorescence); Transfection; CCK8; EdU Staining; Colony Formation Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
upregulated in GC and positively correlated with tumor length, tumor volume, TNM stage and T stage; not associated with overall survival |
| Description: |
CircARID1A is up-regulated in gastric cancer and mainly localizes to the cytoplasm. It binds IGF2BP3 and SLC7A5 mRNA to form a ternary RNA-protein complex that increases SLC7A5 mRNA stability and promotes GC proliferation through AKT/mTOR signaling. |
| Confidence score: |
0.8719 |