| Expression pattern: |
UP |
| Associated gene: |
IL-6, STAT3 |
| Associated microRNA: |
miR-149 |
| Biological function: |
promotes cell proliferation and migration and inhibits apoptosis in gastric cancer cells |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-149, thereby indirectly regulating the IL-6/STAT3 signaling pathway. |
| Biological pathway or process: |
IL-6/STAT3 signaling (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RNase R Treatment; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Transfection; CCK8; Annexin V/PI Flow Cytometry; Wound Healing Assay; Luciferase Reporter Assay; Western Blot |
| Clinical significance: |
High hsa_circ_0017728 expression was closely associated with poor overall survival/disease-free survival, poorer pathological differentiation, higher TNM stage and lymph node metastasis in GC patients. |
| Description: |
hsa_circ_0017728 is up-regulated in gastric carcinoma and promotes proliferation and migration while inhibiting apoptosis. Mechanistically, it functions as a ceRNA sponging miR-149 and thereby activates the IL-6/STAT3 signaling axis; high expression is associated with worse clinicopathologic features and poor survival. |
| Confidence score: |
0.8162 |