| Expression pattern: |
UP |
| Associated gene: |
CEMIP, p-AKT, cyclin D1, cIAP2 |
| Associated microRNA: |
miR-486-5p |
| Biological function: |
promotes hypoxia-induced pulmonary arterial smooth muscle cell (PASMC) proliferation and contributes to pulmonary vascular remodelling in COPD; inhibits apoptosis in hypoxic PASMCs (as reflected by TUNEL). |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-486-5p, relieving miR-486-5p suppression of CEMIP and affecting downstream p-AKT signaling to promote PASMC proliferation under hypoxia. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); PI3K/AKT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; MTT; EdU Staining; TUNEL; Western Blot; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
circ-BPTF is increased in pulmonary arteries of COPD patients and is suggested as a potential biomarker/target for COPD-associated pulmonary vascular remodelling. |
| Description: |
circ-BPTF is up-regulated in pulmonary arteries of COPD patients and is induced by hypoxia in hPASMCs. It promotes hypoxia-driven PASMC proliferation by sponging miR-486-5p, thereby de-repressing CEMIP and enhancing downstream AKT-related pro-proliferative signaling. |
| Confidence score: |
0.753 |