circRNA basic information
circBase ID: hsa_circ_0060927
Name: hsa_circ_CYP24A1
Synonym: circCYP24A1
Host Gene: CYP24A1
Genomic location(hg19): chr20:52773707-52788209:-
Genomic location(hg38): chr20:54157168-54171670:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005580
MONDO name: esophageal squamous cell carcinoma
Disease details: esophageal squamous cell carcinoma / ESCC
Disease DO ID:
 3748
Disease MeSH ID:
 C562729; D000077277
Disease NCIt ID:
 C4024
Disease ICD11 ID:
 1417891145
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

ESCC tissues; normal esophageal tissues; tissue microarray
Cell lines:

TE1; KYSE30; KYSE150; KYSE170
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PKM2, NF-kappaB, CCL5
Associated microRNA: -
Biological function: promotes proliferation, colony formation, migration, invasion and tumor growth in ESCC
Molecular mechanism: circCYP24A1 binds PKM2 to activate NF-kappaB (p65 phosphorylation), increasing CCL5 secretion and promoting ESCC progression
Biological pathway or process:

NF-kappaB (promotes); proliferation (promotes); migration (promotes); invasion (promotes)
Detected method:
Q
H
S
Validation methods:

RNA-seq; RT-qPCR; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; RNA Pull-Down; Western Blot; ELISA; IF (Immunofluorescence); In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

circCYP24A1 expression was significantly upregulated in ESCC tissues; high expression indicates poor prognosis and is an independent prognostic factor
Description:

circCYP24A1 (hsa_circ_0060927), derived from CYP24A1, is up-regulated in ESCC and promotes proliferation, migration, invasion, colony formation, and xenograft tumor growth. Mechanistically, it binds PKM2 to enhance NF-kappaB activation (p65 phosphorylation), increasing CCL5 secretion; high circCYP24A1 is associated with shorter overall survival and serves as a prognostic biomarker/target.
Confidence score:

0.8611
Other information
Title:

circCYP24A1 facilitates esophageal squamous cell carcinoma progression through binding PKM2 to regulate NF-kappaB-induced CCL5 secretion.

Journal: Molecular cancer
Published: 2022
PubMed ID: 36514094
Study type:

combined biological and clinical study
Data availability: -
Code availability: -