circRNA basic information
circBase ID: -
Name: hsa_circ_EPS15
Synonym: circEPS15
Host Gene: EPS15
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005180
MONDO name: Parkinson disease
Disease details: Parkinson's disease / PD
Disease DO ID:
 14330
Disease MeSH ID:
 D010300
Disease NCIt ID:
 C26845
Disease ICD11 ID:
 296066191
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

plasma; peripheral blood samples; midbrain; SNpc; striatum
Cell lines:

SH-SY5Y; HEK-293T; C8; BV2
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
DN
Associated gene: PINK1, PRKN, AGO2, EPS15
Associated microRNA: MIR24-3p / Mir24-3p
Biological function: circEPS15 protects dopaminergic neurons against neurotoxin-induced PD-like neurodegeneration, promotes cell viability/proliferation, inhibits apoptosis, accelerates mitophagy, clears damaged mitochondria, maintains mitochondrial homeostasis, and improves motor behavioral symptoms in PD mouse models.
Molecular mechanism: circEPS15 acts as a MIR24-3p sponge, relieves MIR24-3p-mediated suppression of PINK1, and enhances PINK1-PRKN-dependent mitophagy to improve mitochondrial function and protect dopaminergic neurons.
Biological pathway or process:

ceRNA regulation (other); autophagy (promotes); mitochondrial function (promotes); apoptosis (inhibits); proliferation (promotes); other pathway/process (promotes)
Detected method:
Q
H
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; IF (Immunofluorescence); IHC (Immunohistochemistry); Western Blot; In Vivo Animal Model; ROC Analysis; Bioinformatics Analysis
Clinical significance:

circEPS15 is downregulated in PD patients, distinguishes PD patients from normal controls by ROC analysis, correlates negatively with H-Y stage and UPDRS motor score, and is proposed as a potential biomarker and therapeutic target for PD.
Description:

circEPS15 is downregulated in plasma of PD patients and in MPTP-induced PD mouse models, and lower levels correlate with worse PD motor severity. Functionally, circEPS15 overexpression protects dopaminergic neurons in vitro and in vivo by sponging MIR24-3p, increasing PINK1 expression, and boosting PINK1-PRKN-mediated mitophagy to maintain mitochondrial homeostasis. The study proposes circEPS15 as a potential biomarker and therapeutic target for Parkinson disease.
Confidence score:

0.8792
Other information
Title:

CircEPS15, as a sponge of MIR24-3p ameliorates neuronal damage in Parkinson disease through boosting PINK1-PRKN-mediated mitophagy.

Journal: Autophagy
Published: 2023
PubMed ID: 37014258
Study type:

combined biological and clinical study
Data availability: -
Code availability: -