| Expression pattern: |
UP |
| Associated gene: |
CHMP4B, METTL14, YTHDC1, AGO2 |
| Associated microRNA: |
miR-552-3p |
| Biological function: |
promotes HCC cell proliferation and invasion, inhibits apoptosis, and promotes tumor growth in vivo |
| Molecular mechanism: |
METTL14-mediated m6A modification promotes circFUT8 nuclear export; cytoplasmic circFUT8 acts as a ceRNA sponging miR-552-3p to upregulate CHMP4B; M1 macrophage-derived exosomal miR-628-5p suppresses METTL14, reducing circFUT8 m6A modification and limiting its nuclear export |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes); m6A modification (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Colony Formation Assay; EdU Staining; Transwell Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; Bioinformatics Analysis; MeRIP / MeRIP-seq; Western Blot |
| Clinical significance: |
- |
| Description: |
circFUT8 is up-regulated in HCC cell lines and acts oncogenically by promoting proliferation and invasion while inhibiting apoptosis. Mechanistically, cytoplasmic circFUT8 sponges miR-552-3p to increase CHMP4B, and its m6A modification by METTL14 facilitates nuclear export; M1 macrophage-derived exosomal miR-628-5p represses METTL14 to reduce circFUT8 m6A and limit this ceRNA program, suppressing HCC progression. |
| Confidence score: |
0.8164 |