circRNA basic information
circBase ID: -
Name: hsa_circ_SNX14
Synonym: circSNX14
Host Gene: SNX14
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; adjacent normal tissues; tumor tissues; adjacent non-cancerous tissues; xenograft tumor samples
Cell lines:

Huh7; HepG2; HCCLM3; Hep3B; L02
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: LATS2 mRNA, LATS2, Ago2
Associated microRNA: miR-562
Biological function: Functions as a tumor suppressor; suppresses HCC cell proliferation, colony formation, invasion, angiogenesis, EMT features, and in vivo tumorigenesis.
Molecular mechanism: circSNX14 acts as a molecular sponge for miR-562, thereby maintaining or increasing LATS2 expression through the circSNX14/miR-562/LATS2 axis.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (inhibits); invasion (inhibits); angiogenesis (inhibits); EMT (inhibits); metastasis (inhibits); Hippo/YAP (other)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Transwell Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Survival Analysis
Clinical significance:

Reduced circSNX14 expression was associated with poorer overall survival and with TNM stage and distal node metastasis; circSNX14 may be a prognostic marker.
Description:

circSNX14 is down-regulated in HCC tissues and cell lines, and lower expression is associated with poorer overall survival and more aggressive clinicopathological features. Functionally, circSNX14 suppresses HCC cell proliferation, invasion, angiogenesis, EMT features, and xenograft tumor growth. Mechanistically, it sponges miR-562 and promotes LATS2 expression through the circSNX14/miR-562/LATS2 axis.
Confidence score:

0.8769
Other information
Title:

The circSNX14 functions as a tumor suppressor via the miR-562/ LATS2 pathway in hepatocellular carcinoma cells.

Journal: Journal of molecular histology
Published: 2023
PubMed ID: 37861952
Study type:

combined biological and clinical study
Data availability: The data will be available from corresponding author by reasonable request.
Code availability: -