| Expression pattern: |
DS |
| Associated gene: |
GUCY1A2, STK32A, TTYH2, VDAC3 |
| Associated microRNA: |
hsa-miR-452-3p, hsa-miR-548c-3p, hsa-miR-592, hsa-miR-609, hsa-miR-758-5p |
| Biological function: |
promotes GC cell proliferation, migration and invasion; associated with lymphatic and distal metastasis; potential biomarker for early GC screening and prognosis |
| Molecular mechanism: |
predicted ceRNA/miRNA sponge (TargetScan/miRanda) with multiple MREs; transcriptome sequencing identified downstream target genes after circRNA overexpression |
| Biological pathway or process: |
ceRNA regulation (other); apoptosis (other); immune regulation (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Sanger Sequencing; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; Colony Formation Assay; Transwell Assay; Survival Analysis; ROC Analysis; RNA-seq; Bioinformatics Analysis |
| Clinical significance: |
Tissue hsa_circ_0005927 level correlates with lymphatic metastasis and distal metastasis and with OS/DFS; plasma level increased in early GC and shows screening value (ROC AUC 0.623) |
| Description: |
hsa_circ_0005927 shows abnormal expression in gastric carcinogenesis: it is decreased in HGIEN/GC tissues but increased in plasma of early GC patients. Functional assays indicate it promotes GC cell proliferation, migration, and invasion, and it is predicted to act as a cytoplasmic miRNA sponge (ceRNA) interacting with five miRNAs and affecting hundreds of downstream genes, with potential utility as an early screening and prognostic biomarker. |
| Confidence score: |
0.7623 |