| Expression pattern: |
UP |
| Associated gene: |
COL6A3 mRNA, COL6A3 |
| Associated microRNA: |
miR-3064-5p |
| Biological function: |
Promotes gastric cancer cell proliferation and migration, exacerbates malignancy, and suppresses apoptosis when overexpressed; circCOL6A3 knockdown restricts proliferation and migration and increases apoptosis. |
| Molecular mechanism: |
circCOL6A3 functions as a cytoplasmic ceRNA that sponges miR-3064e5p, thereby relieving miR-3064e5p-mediated suppression of COL6A3 mRNA. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
May provide theoretical grounds and a potential therapeutic target for future clinical diagnosis and treatment of gastric cancer patients. |
| Description: |
circCOL6A3 (hsa_circ_0006401), derived from COL6A3, is up-regulated in gastric cancer cells and acts as an oncogenic circRNA. It is mainly cytoplasmic and functions as a ceRNA by binding miR-3064e5p, thereby derepressing COL6A3 mRNA and promoting proliferation and migration while reducing apoptosis of GC cells. |
| Confidence score: |
0.8118 |