| Expression pattern: |
UP |
| Associated gene: |
PACS2, Argonaute-2 |
| Associated microRNA: |
miR-637 |
| Biological function: |
Facilitates ox-LDL-induced endothelial cell dysfunction by promoting apoptosis and oxidative stress and reducing cell viability and angiogenesis. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-637 to upregulate PACS2 (ceRNA-like regulation), thereby aggravating ox-LDL-induced endothelial injury. |
| Biological pathway or process: |
apoptosis (promotes); angiogenesis (inhibits); oxidative phosphorylation (unclear); mitochondrial function (unclear); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; CCK8; Tube Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
In humans, circ_0002194 upregulation might be used for early screening and clinical diagnosis of patients with AS. |
| Description: |
circ_0002194 is upregulated in ox-LDL-treated human endothelial cells and aggravates endothelial dysfunction. It functions as a sponge for miR-637, relieving miR-637-mediated repression of PACS2, thereby promoting apoptosis and oxidative injury and impairing angiogenic capacity. |
| Confidence score: |
0.7394 |