| Expression pattern: |
UP |
| Associated gene: |
p53 signaling pathway, Forkhead Box O (Foxo) signaling pathways |
| Associated microRNA: |
- |
| Biological function: |
Supports endothelial capillary-like network formation and proliferation in the context of oxidative stress/redox imbalance. |
| Molecular mechanism: |
Predicted circRNA-miRNA-mRNA regulatory network (ceRNA-like) enriched for p53 and Foxo signaling pathways; functional effects supported by circANKRD12 knockdown. |
| Biological pathway or process: |
p53 signaling (other); Foxo (other); proliferation (promotes); angiogenesis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Transfection; Tube Formation Assay; Clinical Sample Validation |
| Clinical significance: |
In CLI skeletal muscle, circANKRD12 showed no significant expression change, but the ANKRD12 circular-to-linear ratio was significantly induced. |
| Description: |
In CLI patient skeletal muscle, circANKRD12 itself was not significantly changed, but the ANKRD12 circular-to-linear ratio was increased. Functionally, circANKRD12 supports endothelial tubulogenesis and proliferation under oxidative stress, and network analysis links it to p53 and Foxo-related stress-response programs. |
| Confidence score: |
0.6722 |