| Expression pattern: |
UP |
| Associated gene: |
CDH5 (VE-cadherin) |
| Associated microRNA: |
hsa-miR-515-5p |
| Biological function: |
Promotes sepsis-induced endothelial hyperpermeability and endothelial barrier disruption; knockdown enhances VE-cadherin expression and improves barrier function. |
| Molecular mechanism: |
Predicted ceRNA mechanism involving hsa_circ_0074158 targeting hsa-miR-515-5p to affect CDH5 (VE-cadherin) and adherens junction/barrier function. |
| Biological pathway or process: |
ceRNA regulation (other); adherens junction (other); migration (other); invasion (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Sanger Sequencing; RNase R Treatment; Clinical Sample Validation; Survival Analysis; Transfection; IF (Immunofluorescence); In Vivo Animal Model; Transwell Assay |
| Clinical significance: |
High hsa_circ_0074158 expression is associated with higher mortality and poor overall survival in sepsis patients; potential biomarker. |
| Description: |
hsa_circ_0074158 is up-regulated in LPS-induced sepsis models and in sepsis patient whole blood. Functionally, its knockdown restores VE-cadherin and reduces endothelial permeability, suggesting it contributes to endothelial barrier disruption, potentially via a predicted hsa-miR-515-5p/CDH5 ceRNA axis. Clinically, high expression is associated with poorer overall survival in sepsis patients. |
| Confidence score: |
0.7548 |