| Expression pattern: |
UP |
| Associated gene: |
VCAM1 |
| Associated microRNA: |
miR-512-5p |
| Biological function: |
promotes proliferation, migration, invasion, colony formation and metastasis; inhibits apoptosis; promotes EMT in intrahepatic cholangiocarcinoma |
| Molecular mechanism: |
Acts as a ceRNA by binding miR-512-5p, thereby upregulating VCAM1 and activating PI3K/AKT signaling. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); EMT (promotes); PI3K/AKT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Transfection; RNA Pull-Down; Luciferase Reporter Assay; MTT; Transwell Assay; Colony Formation Assay; Western Blot; In Vivo Animal Model; TUNEL; IHC (Immunohistochemistry); H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
cMALAT1 expression was analyzed in clinical samples and correlated with clinical features via TCGA. |
| Description: |
circMALAT1 (cMALAT1) is up-regulated in ICC context and acts as an oncogenic circRNA. It sponges miR-512-5p to relieve repression of VCAM1, thereby activating PI3K/AKT signaling and promoting ICC proliferation, EMT-associated migration/invasion, and tumor growth while inhibiting apoptosis. |
| Confidence score: |
0.7597 |