| Expression pattern: |
UP |
| Associated gene: |
HNRNPK, E2F3 mRNA |
| Associated microRNA: |
- |
| Biological function: |
Promotes RB cell proliferation and suppresses cell apoptosis. |
| Molecular mechanism: |
circ-0075804 binds HNRNPK and enhances the mRNA stability of E2F3, thereby promoting RB progression. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); mRNA stability (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Western Blot; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; EdU Staining; Colony Formation Assay; Bioinformatics Analysis |
| Clinical significance: |
circ-0075804 was suggested as a potential therapeutic target for RB patients. |
| Description: |
circ-0075804 is up-regulated in retinoblastoma cells and primary RB tissues. It promotes RB cell proliferation and suppresses apoptosis by binding the RBP HNRNPK and enhancing E2F3 mRNA stability, suggesting potential therapeutic relevance. |
| Confidence score: |
0.8331 |