| Expression pattern: |
UP |
| Associated gene: |
MCAM |
| Associated microRNA: |
miR-640 |
| Biological function: |
promotes OS cell growth and migration; promotes tumor growth in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge/ceRNA to bind miR-640 and thereby releases MCAM from miR-640-mediated suppression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ROC Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model |
| Clinical significance: |
Diagnostic potentials of levels of circ_0097271 for OS were evaluated by ROC curve analysis... Area under the curve was 0.9744 |
| Description: |
circ_0097271 is up-regulated in osteosarcoma tissues and cell lines and mainly localized in the cytoplasm. Its knockdown inhibits proliferation and migration in vitro and suppresses xenograft tumor growth in vivo, at least partly by sponging miR-640 to de-repress the downstream target MCAM. |
| Confidence score: |
0.8025 |