| Expression pattern: |
UP |
| Associated gene: |
PDK1 / Pdk1 mRNA |
| Associated microRNA: |
hsa-miR-888, hsa-miR-890, hsa-miR-892a, hsa-miR-892b |
| Biological function: |
promotes proliferation, migration, invasion, tumorigenicity, and metastasis of breast cancer/TNBC cells |
| Molecular mechanism: |
acts as a ceRNA/miRNA sponge for the miR-888 family to regulate PDK1 expression |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Bioinformatics Analysis; Transfection; circRIP; RNA Pull-Down; Luciferase Reporter Assay; Western Blot; CCK8; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining |
| Clinical significance: |
circRAD54L2 might act as a prognostic biomarker and therapeutic target for breast cancer |
| Description: |
circRAD54L2 (hsa_circ_0001306), derived from RAD54L2 exons 1-4, is up-regulated in breast cancer/TNBC cell lines. It promotes proliferation, migration, invasion and metastasis by sponging the miR-888 family (hsa-miR-888/miR-890/miR-892a/miR-892b) to derepress PDK1 in a ceRNA manner. |
| Confidence score: |
0.8118 |