| Expression pattern: |
UP |
| Associated gene: |
NFAT5 |
| Associated microRNA: |
miR-34a-5p |
| Biological function: |
Promotes NSCLC cell proliferation and invasion and inhibits apoptosis; its knockdown restrains tumor growth in vivo and enhances the anticancer effect of 2-MeOE2. |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-34a-5p to regulate NFAT5 expression; involved in 2-MeOE2 antitumor effects. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
circ_0010235 might be a diagnostic and prognostic marker in NSCLC. |
| Description: |
circ_0010235 is up-regulated in NSCLC tissues and cells and promotes malignant phenotypes. It sponges miR-34a-5p to upregulate NFAT5, and its knockdown suppresses xenograft tumor growth and enhances the antitumor effect of 2-MeOE2. |
| Confidence score: |
0.7487 |