| Expression pattern: |
UP |
| Associated gene: |
IGF2BP2, IGF2 mRNA, IGF2 |
| Associated microRNA: |
- |
| Biological function: |
Promotes CRC cell viability, migration, invasion, EMT progression, and tumor growth in vivo. |
| Molecular mechanism: |
circ_0000847 directly binds the RBP IGF2BP2 and enhances IGF2 mRNA stability, increasing IGF2 expression to promote CRC progression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); mRNA stability (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0000847 could serve as an innovative and potentially pivotal target for the development of CRC therapies. |
| Description: |
hsa_circ_0000847 is up-regulated in colorectal cancer and acts as an oncogenic circRNA. It promotes CRC cell viability, migration, invasion, EMT, and xenograft tumor growth by binding IGF2BP2 and stabilizing IGF2 mRNA, suggesting potential therapeutic relevance. |
| Confidence score: |
0.8237 |