circRNA basic information
circBase ID: hsa_circ_0000390
Name: hsa_circ_FGD4
Synonym: Circ_0000390
Host Gene: FGD4
Genomic location(hg19): chr12:32760890-32764217:+
Genomic location(hg38): chr12:32607956-32611283:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissue; normal (para-carcinoma) tissue; CRC xenograft tumors
Cell lines:

CACO-2; HCT116; Lovo; SW620; SW480; RKO
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: METTL3, Notch1, Hes1, Vimentin, N-cadherin, E-cadherin
Associated microRNA: -
Biological function: circ_0000390 acts as an anti-tumor factor in colorectal cancer; its overexpression suppresses CRC cell proliferation, viability, migration, invasion, EMT-related protein changes, and xenograft tumor growth induced by METTL3.
Molecular mechanism: METTL3 downregulates circ_0000390, while circ_0000390 suppresses CRC progression by downregulating Notch1 and the Notch1/Hes1 pathway with associated EMT marker changes.
Biological pathway or process:

Notch (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); m6A modification (other)
Detected method:
Q
Validation methods:

Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; TUNEL
Clinical significance:

The study suggests circ_0000390 may provide a basis for diagnosis, therapy, and prognosis assessment of CRC, but no direct diagnostic or prognostic performance was reported.
Description:

hsa_circ_0000390 is downregulated in colorectal cancer tissues and functions as a tumor-suppressive circRNA. Its overexpression inhibits CRC cell proliferation, migration, invasion, EMT-related changes, and xenograft tumor growth, at least partly by reducing Notch1/Hes1 signaling; METTL3-mediated downregulation of circ_0000390 promotes malignant CRC phenotypes.
Confidence score:

0.6285
Other information
Title:

METTL3-mediated Hsa_circ_0000390 downregulation enhances the proliferation, migration, and invasion of colorectal cancer cells by increasing Notch1 expression.

Journal: Human cell
Published: 2023
PubMed ID: 36600025
Study type:

combined biological and clinical study
Data availability: Data available within the article or its supplementary materials; https:// doi. org/ 10. 1007/ s13577-022-00835-w
Code availability: -