| Expression pattern: |
UP |
| Associated gene: |
TGF-beta1, tumor-derived exosomes |
| Associated microRNA: |
miR-142-3p, miR-506-3p |
| Biological function: |
promotes CRC cell proliferation, migration and invasion; inhibits apoptosis; promotes differentiation of N1 to N2 neutrophils |
| Molecular mechanism: |
circPACRGL acts as a miRNA sponge for miR-142-3p and miR-506-3p, thereby facilitating TGF-beta1 expression and promoting CRC progression; exosomal transfer contributes to these effects |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); immune regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; CCK8; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model |
| Clinical significance: |
a valuable marker for CRC treatment |
| Description: |
In colorectal cancer, exosome-associated circPACRGL is upregulated in CRC cells after tumor-derived exosome stimulation. It promotes proliferation, migration/invasion and suppresses apoptosis by sponging miR-142-3p and miR-506-3p to increase TGF-beta1 expression, and it also promotes N1-to-N2 neutrophil differentiation via the same axis. |
| Confidence score: |
0.6181 |