| Expression pattern: |
UP |
| Associated gene: |
SNAIL, AGO2 |
| Associated microRNA: |
miR-370-3p |
| Biological function: |
Promotes NSCLC progression by promoting viability, invasion, migration, metastasis, and inducing EMT. |
| Molecular mechanism: |
circSWT1 functions as a ceRNA sponge for miR-370-3p, relieving repression of SNAIL and thereby inducing EMT and NSCLC progression. |
| Biological pathway or process: |
EMT (promotes); migration (promotes); invasion (promotes); metastasis (promotes); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; circRIP; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circSWT1 expression predicts poor prognosis (worse overall survival and higher postoperative recurrence) and is an independent predictor of prognosis in NSCLC. |
| Description: |
In NSCLC, circSWT1 (hsa_circ_0004689) is upregulated and promotes tumor growth, invasion, migration, metastasis and EMT. It acts as a miR-370-3p sponge to derepress SNAIL, and SNAIL knockout reverses circSWT1-driven phenotypes. High circSWT1 expression is associated with poor prognosis. |
| Confidence score: |
0.8815 |