| Expression pattern: |
DN |
| Associated gene: |
P21CIP1, Ago2 |
| Associated microRNA: |
miR-370-3p |
| Biological function: |
inhibits proliferation; induces G0/G1 cell cycle arrest |
| Molecular mechanism: |
ceRNA sponge mechanism: circITGA7 binds miR-370-3p and regulates P21CIP1 expression |
| Biological pathway or process: |
proliferation (inhibits); cell cycle (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Sanger Sequencing; divergent primers PCR; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Cell Cycle Assay; In Vivo Animal Model |
| Clinical significance: |
circITGA7 expression was not remarkably correlated with pathological stage, Gleason score, and blood PSA level |
| Description: |
circITGA7 is down-regulated in prostate cancer tissues and cell lines and suppresses PCa cell proliferation by inducing G0/G1 arrest. It acts as a ceRNA by sponging miR-370-3p, thereby increasing the expression of P21CIP1; the anti-proliferative effect can be rescued by miR-370-3p overexpression. |
| Confidence score: |
0.7763 |