| Expression pattern: |
UP |
| Associated gene: |
MAPK7, HK2 |
| Associated microRNA: |
miR-372-3p |
| Biological function: |
promotes glycolysis, proliferation, migration, invasion and EMT, and inhibits apoptosis in osteosarcoma cells; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circ_0001721 sponges miR-372-3p to upregulate MAPK7 |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model |
| Clinical significance: |
High circ_0001721 expression was associated with tumor size and pulmonary metastasis and predicted poorer overall survival in OS patients. |
| Description: |
circ_0001721 is upregulated in osteosarcoma and primarily localized in the cytoplasm. It promotes glycolysis, proliferation, migration, invasion and EMT while inhibiting apoptosis by sponging miR-372-3p to increase MAPK7 expression, and its high expression is associated with worse overall survival. |
| Confidence score: |
0.7588 |