| Expression pattern: |
DN |
| Associated gene: |
SFRP1, WNT/beta‐catenin, Ago2 |
| Associated microRNA: |
miR‐576‐5p |
| Biological function: |
inhibits migration, invasion, and proliferation; promotes apoptosis; suppresses tumor growth and intraperitoneal metastasis |
| Molecular mechanism: |
ceRNA (miRNA sponge) mechanism: hcR1445 binds miR-576-5p to relieve repression of SFRP1, thereby inhibiting WNT/beta-catenin signaling |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Patients who had higher levels of hcR1445 had longer survival times comparing to those who had lower hcR1445; low hcR1445 production is closely associated with poor survival |
| Description: |
hsa_circ_0001445 (hcR1445/circSMARCA5) is down-regulated in ovarian cancer and functions as a tumor suppressor. It sponges miR-576-5p to increase SFRP1, thereby inhibiting WNT/beta-catenin signaling and reducing proliferation, migration, invasion and in vivo metastasis while promoting apoptosis; higher hcR1445 correlates with longer patient survival. |
| Confidence score: |
0.8712 |