| Expression pattern: |
DN |
| Associated gene: |
RUNX3, beta-catenin |
| Associated microRNA: |
miR-558 |
| Biological function: |
inhibits gastric cancer cell proliferation, migration, invasion, EMT and tumorigenesis |
| Molecular mechanism: |
Acts as a miR-558 sponge to upregulate RUNX3 and inactivate beta-catenin signaling. |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
Low expression of circREPS2 was associated with a higher tumor-node-metastasis (TNM) stage, poor tumor differentiation, and larger tumor size in GC patients. |
| Description: |
circREPS2 (hsa_circ_0139996) is down-regulated in gastric cancer tissues and cell lines and functions as a tumor suppressor. It sponges miR-558 to upregulate RUNX3, thereby inhibiting Wnt/beta-catenin signaling and suppressing proliferation, migration, invasion, EMT, and xenograft tumor growth. |
| Confidence score: |
0.852 |