circRNA basic information
circBase ID: hsa_circ_0000043
Name: -
Synonym: circRNA_0000043
Host Gene: -
Genomic location(hg19): chr1:31465236-31468067:-
Genomic location(hg38): chr1:30992389-30995220:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tissues; normal breast tissues; paracancerous tissue
Cell lines:

MDA-MB-231; MCF7; ZR751; MCF-10A
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: Smad3
Associated microRNA: miR-136
Biological function: Promotes breast cancer cell proliferation, migration, invasion, and epithelial-mesenchymal transition.
Molecular mechanism: circ_0000043 functions as a ceRNA by sponging miR-136, thereby up-regulating Smad3 expression and promoting breast cancer progression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; IHC (Immunohistochemistry); Transfection; CCK8; Wound Healing Assay; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Bioinformatics Analysis
Clinical significance:

High circ_0000043 expression was associated with lymph node metastasis, poor differentiation of tumor tissues, poor prognosis, and may be a potential biomarker and therapeutic target for breast cancer.
Description:

circ_0000043 is up-regulated in breast cancer tissues and cell lines and is associated with adverse clinicopathological features. Functionally, it promotes breast cancer cell proliferation, migration, invasion, and EMT. Mechanistically, circ_0000043 sponges miR-136 to increase Smad3 expression, forming a circ_0000043/miR-136/Smad3 regulatory axis.
Confidence score:

0.7537
Other information
Title:

Circ_0000043 promotes the proliferation, migration, invasiveness, and epithelial-mesenchymal transition in breast cancer cells via the miR-136-Smad3 axis.

Journal: Biochemistry and cell biology = Biochimie et biologie cellulaire
Published: 2021
PubMed ID: 33043682
Study type:

combined biological and clinical study
Data availability: The data used to support the findings of this study are available from the corresponding author upon request.
Code availability: -